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| Registros recuperados: 30 | |
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| Le Moullac, Gilles; Phillip De Laborie, Loic; Saulnier, Denis; Goarant, Cyrille; Dehasque, Marleen. |
| Anderson (1992) y Raa (1996) han escrito impresionantes críticas acerca del uso de inmunoestimulantes en acuacultura. La mayoría de los trabajos citados se refieren principalmente al cultivo de peces y pocos acerca del camarón. Así que este trabajo está enfocado al camarón y las pocas pruebas publicadas. Varios de los inmunoestimulantes y su manera de actuar sobre el sistema inmune en camarones se describen brevemente, así como los métodos usados para administrar las sustancias inmunoestimulatorias para camarón. La dificultad para experimentar en este campo está señalada con algunos ejemplos. |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00029/14011/11197.pdf |
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| Goarant, Cyrille; Merien, F. |
| Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular... |
| Tipo: Text |
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| Goarant, Cyrille. |
| Le suivi épidémiologique de Vibrio penaeicida a débuté en février 1998 sur quatre fermes et quatre écloseries du Territoire. Cette fiche biotechnique présente les résultats préliminaires issus de l' étude d'un élevage sur le bassin 9 de la Station d'Aquaculture de Saint Vincent. Ce suivi s'est accompagné d'un suivi des comptages hémocytaires des crevettes en intermue de ce bassin, dont les résultats seront présentés dans une fiche biotechnique séparée. Un important pic de mortalité a été observé dans ce bassin. Les crevettes moribondes étudiées en début du pic de mortalité étaient toutes atteintes d'une vibriose à Vibrio penaeicida. Par la suite, l'ADN de Vibrio penaeicida n'a pas systématiquement été détecté dans les crevettes examinées au moment de la... |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00274/38543/37065.pdf |
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| Goarant, Cyrille. |
| Dans le cadre du suivi épidémiologique de Vibrio penaeicida dans les élevages de crevettes du Territoire, il importait de considérer l'éventualité d'une transmission pseudo-verticale du pathogène, c'est-à-dire des géniteurs aux larves et post-larves. Ce suivi a concerné des productions de quatre écloseries du Territoire (Ecloserie du Nord, Ecloserie de Mara, Ecloserie de Montagnès et Ecloserie de la SASV) à différentes saisons, et sur différents stades de développement. Le portage de V. penaeicida est confirmé sur les géniteurs, mais la transmission pseudo-verticale semble exclue au vu des résultats de ce suivi. |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00274/38542/37062.pdf |
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| Goarant, Cyrille; Ansquer, Dominique; Herlin, Jose; Domalain, David; Imbert, Frederic; De Decker, Sophie. |
| The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but... |
| Tipo: Text |
Palavras-chave: Epidemiology; Vibrio; Diseases and their control; Shrimp. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1110.pdf |
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| Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B. |
| Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida... |
| Tipo: Text |
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf |
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| Goarant, Cyrille. |
| Une étude de typage moléculaire par AP-PCR de souches de Vibrio impliqués dans des épisodes de mortalité d'élevages de crevettes en Nouvelle-Calédonie (Syndrome 93) et au Japon a été réalisée fin 1996 avec le concours de l' Institut Pasteur de Nouvelle-Calédonie. Elle nous a permis une identification rapide des isolats en terme de génospecies ainsi que des discriminations d' intérêt épidémiologique entre les isolats du génospecies majoritaire. Ainsi, des sous-groupes ont été identifiés au sein de l' espéce majoritaire : V. penaeicida. Certains de ces sous-groupes correspondent à des topotypes et permettent de discriminer les isolats du Japon de ceux de Nouvelle-Calédonie, ainsi que ceux de deux baies différentes de Nouvelle Calédonie, distinctes de 50 km... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00274/38541/37061.pdf |
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| Le Roux, Frederique; Labreuche, Yannick; Davis, Brigid M.; Iqbal, Naeem; Mangenot, Sophie; Goarant, Cyrille; Mazel, Didier; Waldor, Matthew K.. |
| Vibrioses are the predominant bacterial infections in marine shrimp farms. Vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp Litopenaeus stylirostris in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic V. nigripulchritudo isolates from New Caledonia all cluster into a monophyletic clade and contain a small plasmid, pB1067. Here, we report that a large plasmid, pA1066 (247 kb), can also serve as a marker for virulent V. nigripulchritudo, and that an ancestral version of this plasmid was likely acquired prior to other virulence-linked markers. Additionally, we demonstrate that... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14196/11469.pdf |
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| Registros recuperados: 30 | |
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